rabbit anti carma1 (ProSci Incorporated)
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Rabbit Anti Carma1, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+carma1/CARMA1+Antibody/10__1128_slash_mcb__00876___15-59-19-21
Average 90 stars, based on 12 article reviews
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Western Blot:Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: .. Cells were boiled in SDS resolved on a 12% gel and western blotted with mouse anti-300 RNF181 (Santa Cruz sc-101120), Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: pLKO.1-based lentiviruses expressing short hairpin RNA (shRNA) against a nontarget (NT) control (shNT control; sense sequence, 5′-CAACAAGATGAAGAGCACCAA-3′; loop sequence, 5′-CTCGAG-3′; catalog number SHC002; Sigma), two different shRNAs targeting RNF181, shRNF181-2 (sense sequence, 5′-TGGAGCTCGCAAGGTCACTTT-3′; loop sequence, 5′-CTCGAG-3′) and shRNF181-4 (sense sequence, 5′-CCTCTGCGTCTTCCTTATTAA-3′; loop sequence, 5′-CTCGAG-3′), or an shRNA targeting CARD11 (sense sequence, 5′-TGGTCAAGAAGCTGACGATTC-3′; loop sequence, 5′-TTCAAGAGA-3′) were packaged in HEK293T cells, and Jurkat T cells were infected and selected with 0.5 μg/ml puromycin (Sigma) as previously described ( 16 , 28 ). .. Knockdown was assayed by Western blotting using mouse anti-RNF181 (catalog number sc-101120; Santa Cruz), rabbit anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; catalog number D16H11; Cell Signaling), Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: 161 8 162 Lentivirus-mediated Stable Knockdown lines 163 pLKO.1-based lentiviruses expressing shNT (Sigma SHC002) (sense sequence, 5’-164 CAACAAGATGAAGAGCACCAA-3’ loop sequence 5’-CTCGAG-3’), two different shRNAs 165 targeting RNF181, shRNF181-2 (sense sequence, 5’ TGGAGCTCGCAAGGTCACTTT-3’ loop 166 sequence 5’-CTCGAG-3’) and shRNF181-4 (sense sequence, 5’- 167 CCTCTGCGTCTTCCTTATTAA-3’ loop sequence 5’-CTCGAG-3’), or a shRNA targeting 168 CARD11 (sense sequence, 5’-TGGTCAAGAAGCTGACGATTC-3’ loop sequence, 5’-169 TTCAAGAGA-3’) were packaged in HEK293T cells and Jurkat T cells were infected and 170 selected with 0.5 μg/ml puromycin (Sigma) as previously described (16, 28). .. Knockdown was 171 assayed by western blot using mouse anti-RNF181 (Santa Cruz sc-101120), rabbit anti-GAPDH 172 (Cell Signaling D16H11), Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: .. Cells were boiled in SDS and resolved on a 12% gel, and Western blotting was performed with mouse anti-RNF181 (catalog number sc-101120; Santa Cruz), Isolation:Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: .. CARD11 was detected with Bioluminescence Resonance Energy Transfer:Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: .. CARD11 was detected with Cloning:Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: .. CARD11 was detected with Knockdown:Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: pLKO.1-based lentiviruses expressing short hairpin RNA (shRNA) against a nontarget (NT) control (shNT control; sense sequence, 5′-CAACAAGATGAAGAGCACCAA-3′; loop sequence, 5′-CTCGAG-3′; catalog number SHC002; Sigma), two different shRNAs targeting RNF181, shRNF181-2 (sense sequence, 5′-TGGAGCTCGCAAGGTCACTTT-3′; loop sequence, 5′-CTCGAG-3′) and shRNF181-4 (sense sequence, 5′-CCTCTGCGTCTTCCTTATTAA-3′; loop sequence, 5′-CTCGAG-3′), or an shRNA targeting CARD11 (sense sequence, 5′-TGGTCAAGAAGCTGACGATTC-3′; loop sequence, 5′-TTCAAGAGA-3′) were packaged in HEK293T cells, and Jurkat T cells were infected and selected with 0.5 μg/ml puromycin (Sigma) as previously described ( 16 , 28 ). .. Knockdown was assayed by Western blotting using mouse anti-RNF181 (catalog number sc-101120; Santa Cruz), rabbit anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; catalog number D16H11; Cell Signaling), Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181 Article Snippet: 161 8 162 Lentivirus-mediated Stable Knockdown lines 163 pLKO.1-based lentiviruses expressing shNT (Sigma SHC002) (sense sequence, 5’-164 CAACAAGATGAAGAGCACCAA-3’ loop sequence 5’-CTCGAG-3’), two different shRNAs 165 targeting RNF181, shRNF181-2 (sense sequence, 5’ TGGAGCTCGCAAGGTCACTTT-3’ loop 166 sequence 5’-CTCGAG-3’) and shRNF181-4 (sense sequence, 5’- 167 CCTCTGCGTCTTCCTTATTAA-3’ loop sequence 5’-CTCGAG-3’), or a shRNA targeting 168 CARD11 (sense sequence, 5’-TGGTCAAGAAGCTGACGATTC-3’ loop sequence, 5’-169 TTCAAGAGA-3’) were packaged in HEK293T cells and Jurkat T cells were infected and 170 selected with 0.5 μg/ml puromycin (Sigma) as previously described (16, 28). .. Knockdown was 171 assayed by western blot using mouse anti-RNF181 (Santa Cruz sc-101120), rabbit anti-GAPDH 172 (Cell Signaling D16H11), |
