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rabbit anti carma1  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated rabbit anti carma1
    Rabbit Anti Carma1, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+carma1/CARMA1+Antibody/10__1128_slash_mcb__00876___15-59-19-21
    Average 90 stars, based on 12 article reviews
    rabbit anti carma1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: .. Cells were boiled in SDS resolved on a 12% gel and western blotted with mouse anti-300 RNF181 (Santa Cruz sc-101120), rabbit anti-CARMA1 (ProSci 3189), rabbit anti-Bcl10 (Santa 301 Cruz sc-5611), and rabbit anti-GAPDH (Cell Signaling D16H11), rabbit anti-IKKγ (Santa Cruz 302 sc-8330), goat anti-IKKβ (Santa Cruz sc-7329) mouse anti-IKKα (Santa Cruz sc-7606), rabbit 303 anti-IκB-α (Santa Cruz sc-371), mouse anti-p-IκB-α (Cell Signaling 9246S), and rabbit anti-p65 304 (Santa Cruz sc-372). ..

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: pLKO.1-based lentiviruses expressing short hairpin RNA (shRNA) against a nontarget (NT) control (shNT control; sense sequence, 5′-CAACAAGATGAAGAGCACCAA-3′; loop sequence, 5′-CTCGAG-3′; catalog number SHC002; Sigma), two different shRNAs targeting RNF181, shRNF181-2 (sense sequence, 5′-TGGAGCTCGCAAGGTCACTTT-3′; loop sequence, 5′-CTCGAG-3′) and shRNF181-4 (sense sequence, 5′-CCTCTGCGTCTTCCTTATTAA-3′; loop sequence, 5′-CTCGAG-3′), or an shRNA targeting CARD11 (sense sequence, 5′-TGGTCAAGAAGCTGACGATTC-3′; loop sequence, 5′-TTCAAGAGA-3′) were packaged in HEK293T cells, and Jurkat T cells were infected and selected with 0.5 μg/ml puromycin (Sigma) as previously described ( 16 , 28 ). .. Knockdown was assayed by Western blotting using mouse anti-RNF181 (catalog number sc-101120; Santa Cruz), rabbit anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; catalog number D16H11; Cell Signaling), rabbit anti-CARMA1 (catalog number 3189; ProSci), rabbit anti-Bcl10 (catalog number sc-5611; Santa Cruz), rabbit anti-MALT1 (catalog number 1664-1; Epitomics), rabbit anti-IKKγ (catalog number sc-8330; Santa Cruz), goat anti-IKKβ (catalog number sc-7329; Santa Cruz), mouse anti-IKKα (catalog number sc-7606; Santa Cruz), rabbit anti-p-IKKα/β (catalog number 2681S; Cell Signaling), and rabbit anti-p65 (catalog number sc-372; Santa Cruz). ..

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: 161 8 162 Lentivirus-mediated Stable Knockdown lines 163 pLKO.1-based lentiviruses expressing shNT (Sigma SHC002) (sense sequence, 5’-164 CAACAAGATGAAGAGCACCAA-3’ loop sequence 5’-CTCGAG-3’), two different shRNAs 165 targeting RNF181, shRNF181-2 (sense sequence, 5’ TGGAGCTCGCAAGGTCACTTT-3’ loop 166 sequence 5’-CTCGAG-3’) and shRNF181-4 (sense sequence, 5’- 167 CCTCTGCGTCTTCCTTATTAA-3’ loop sequence 5’-CTCGAG-3’), or a shRNA targeting 168 CARD11 (sense sequence, 5’-TGGTCAAGAAGCTGACGATTC-3’ loop sequence, 5’-169 TTCAAGAGA-3’) were packaged in HEK293T cells and Jurkat T cells were infected and 170 selected with 0.5 μg/ml puromycin (Sigma) as previously described (16, 28). .. Knockdown was 171 assayed by western blot using mouse anti-RNF181 (Santa Cruz sc-101120), rabbit anti-GAPDH 172 (Cell Signaling D16H11), rabbit anti-CARMA1 (ProSci 3189), rabbit anti-Bcl10 (Santa Cruz sc-173 5611), rabbit anti-MALT1 (Epitomics 1664-1), rabbit anti-IKKγ (Santa Cruz sc-8330), goat anti-174 IKKβ (Santa Cruz sc-7329) mouse anti-IKKα (Santa Cruz sc-7606), rabbit anti-p-IKKα/β (Cell 175 Signaling 2681S),and rabbit anti-p65 (Santa Cruz sc-372). ..

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: .. Cells were boiled in SDS and resolved on a 12% gel, and Western blotting was performed with mouse anti-RNF181 (catalog number sc-101120; Santa Cruz), rabbit anti-CARMA1 (catalog number 3189; ProSci), rabbit anti-Bcl10 (catalog number sc-5611; Santa Cruz), rabbit anti-GAPDH (catalog number D16H11; Cell Signaling), rabbit anti-IKKγ (catalog number sc-8330; Santa Cruz), goat anti-IKKβ (catalog number sc-7329; Santa Cruz), mouse anti-IKKα (catalog number sc-7606; Santa Cruz), rabbit anti-IκBα (catalog number sc-371; Santa Cruz), mouse anti-phosphorylated IκBα (catalog number 9246S; Cell Signaling), and rabbit anti-p65 (catalog number sc-372; Santa Cruz). ..

    Isolation:

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: .. CARD11 was detected with rabbit anti-CARMA1 (catalog number 3189; ProSci), and RNF181 was detected with mouse anti-RNF181 (catalog number sc-101120; Santa Cruz). fig ft0 fig mode=article f1 FIG 1 caption a4 Isolation of RNF181 as a CARD11 interactor in BRET-based interaction cloning. (A) Detection of a protein-protein interaction in live cells by BRET between the appropriate fusions of bait and prey proteins. (B) Clones C1 to C7 from pool C were assayed ... For human CD4 + T cell immunoprecipitations, peripheral blood mononuclear cells were isolated from leukocyte packs (New York Blood Center) via Ficoll gradient separation. .. CD4 + T cells were then purified to >90% by positive selection with a human CD4 + MultiSort kit (catalog number 130-055-101; Miltenyi Biotec).

    Bioluminescence Resonance Energy Transfer:

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: .. CARD11 was detected with rabbit anti-CARMA1 (catalog number 3189; ProSci), and RNF181 was detected with mouse anti-RNF181 (catalog number sc-101120; Santa Cruz). fig ft0 fig mode=article f1 FIG 1 caption a4 Isolation of RNF181 as a CARD11 interactor in BRET-based interaction cloning. (A) Detection of a protein-protein interaction in live cells by BRET between the appropriate fusions of bait and prey proteins. (B) Clones C1 to C7 from pool C were assayed ... For human CD4 + T cell immunoprecipitations, peripheral blood mononuclear cells were isolated from leukocyte packs (New York Blood Center) via Ficoll gradient separation. .. CD4 + T cells were then purified to >90% by positive selection with a human CD4 + MultiSort kit (catalog number 130-055-101; Miltenyi Biotec).

    Cloning:

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: .. CARD11 was detected with rabbit anti-CARMA1 (catalog number 3189; ProSci), and RNF181 was detected with mouse anti-RNF181 (catalog number sc-101120; Santa Cruz). fig ft0 fig mode=article f1 FIG 1 caption a4 Isolation of RNF181 as a CARD11 interactor in BRET-based interaction cloning. (A) Detection of a protein-protein interaction in live cells by BRET between the appropriate fusions of bait and prey proteins. (B) Clones C1 to C7 from pool C were assayed ... For human CD4 + T cell immunoprecipitations, peripheral blood mononuclear cells were isolated from leukocyte packs (New York Blood Center) via Ficoll gradient separation. .. CD4 + T cells were then purified to >90% by positive selection with a human CD4 + MultiSort kit (catalog number 130-055-101; Miltenyi Biotec).

    Knockdown:

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: pLKO.1-based lentiviruses expressing short hairpin RNA (shRNA) against a nontarget (NT) control (shNT control; sense sequence, 5′-CAACAAGATGAAGAGCACCAA-3′; loop sequence, 5′-CTCGAG-3′; catalog number SHC002; Sigma), two different shRNAs targeting RNF181, shRNF181-2 (sense sequence, 5′-TGGAGCTCGCAAGGTCACTTT-3′; loop sequence, 5′-CTCGAG-3′) and shRNF181-4 (sense sequence, 5′-CCTCTGCGTCTTCCTTATTAA-3′; loop sequence, 5′-CTCGAG-3′), or an shRNA targeting CARD11 (sense sequence, 5′-TGGTCAAGAAGCTGACGATTC-3′; loop sequence, 5′-TTCAAGAGA-3′) were packaged in HEK293T cells, and Jurkat T cells were infected and selected with 0.5 μg/ml puromycin (Sigma) as previously described ( 16 , 28 ). .. Knockdown was assayed by Western blotting using mouse anti-RNF181 (catalog number sc-101120; Santa Cruz), rabbit anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; catalog number D16H11; Cell Signaling), rabbit anti-CARMA1 (catalog number 3189; ProSci), rabbit anti-Bcl10 (catalog number sc-5611; Santa Cruz), rabbit anti-MALT1 (catalog number 1664-1; Epitomics), rabbit anti-IKKγ (catalog number sc-8330; Santa Cruz), goat anti-IKKβ (catalog number sc-7329; Santa Cruz), mouse anti-IKKα (catalog number sc-7606; Santa Cruz), rabbit anti-p-IKKα/β (catalog number 2681S; Cell Signaling), and rabbit anti-p65 (catalog number sc-372; Santa Cruz). ..

    Article Title: Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
    Article Snippet: 161 8 162 Lentivirus-mediated Stable Knockdown lines 163 pLKO.1-based lentiviruses expressing shNT (Sigma SHC002) (sense sequence, 5’-164 CAACAAGATGAAGAGCACCAA-3’ loop sequence 5’-CTCGAG-3’), two different shRNAs 165 targeting RNF181, shRNF181-2 (sense sequence, 5’ TGGAGCTCGCAAGGTCACTTT-3’ loop 166 sequence 5’-CTCGAG-3’) and shRNF181-4 (sense sequence, 5’- 167 CCTCTGCGTCTTCCTTATTAA-3’ loop sequence 5’-CTCGAG-3’), or a shRNA targeting 168 CARD11 (sense sequence, 5’-TGGTCAAGAAGCTGACGATTC-3’ loop sequence, 5’-169 TTCAAGAGA-3’) were packaged in HEK293T cells and Jurkat T cells were infected and 170 selected with 0.5 μg/ml puromycin (Sigma) as previously described (16, 28). .. Knockdown was 171 assayed by western blot using mouse anti-RNF181 (Santa Cruz sc-101120), rabbit anti-GAPDH 172 (Cell Signaling D16H11), rabbit anti-CARMA1 (ProSci 3189), rabbit anti-Bcl10 (Santa Cruz sc-173 5611), rabbit anti-MALT1 (Epitomics 1664-1), rabbit anti-IKKγ (Santa Cruz sc-8330), goat anti-174 IKKβ (Santa Cruz sc-7329) mouse anti-IKKα (Santa Cruz sc-7606), rabbit anti-p-IKKα/β (Cell 175 Signaling 2681S),and rabbit anti-p65 (Santa Cruz sc-372). ..



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    FIGURE 2. Normal expression and function of c-REL and IkBNS in the absence of the other factor. (A) Western blot analysis of NF-kB activation in anti- CD3 stimulated (10 mg/ml) total cells from peripheral lymph nodes of wild-type, IkBNS-deficient, c-REL–deficient, and DKO mice. Expression of <t>CARMA1,</t> c-REL, and IkBNS was analyzed along with NF-kB activation via the presence of phosphorylated and total IkBa. The experiment shown is representative of two independent experiments. (B) CD4+ T cells were purified by FACS, expanded for 7 d in vitro, and stimulated with 1 mM ionomycin and 10 ng/ml PMA for 4 h. Subsequently, nuclear and cytoplasmic fractions were analyzed for the presence of c-REL and IkBNS by Western blotting. ERK and LaminB served as controls for loading and fraction purity. The experiment shown is representative of three independent experiments. (C) CD4+ T cells were isolated from lymph nodes and spleens of wild-type, IkBNS-deficient, and c-REL–deficient mice, and stimulated with 10 ng/ml PMA and 1 mM ionomycin for 8 h. ChIP was performed with anti–c-REL Ab, anti-IkBNS Ab, and control rabbit IgG (ctr IgG). Input chromatin and water were used as positive and negative controls, respectively. The experiment shown is representative of two independent experiments.
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    ProSci Incorporated rabbit anti carma1
    FIGURE 2. Normal expression and function of c-REL and IkBNS in the absence of the other factor. (A) Western blot analysis of NF-kB activation in anti- CD3 stimulated (10 mg/ml) total cells from peripheral lymph nodes of wild-type, IkBNS-deficient, c-REL–deficient, and DKO mice. Expression of <t>CARMA1,</t> c-REL, and IkBNS was analyzed along with NF-kB activation via the presence of phosphorylated and total IkBa. The experiment shown is representative of two independent experiments. (B) CD4+ T cells were purified by FACS, expanded for 7 d in vitro, and stimulated with 1 mM ionomycin and 10 ng/ml PMA for 4 h. Subsequently, nuclear and cytoplasmic fractions were analyzed for the presence of c-REL and IkBNS by Western blotting. ERK and LaminB served as controls for loading and fraction purity. The experiment shown is representative of three independent experiments. (C) CD4+ T cells were isolated from lymph nodes and spleens of wild-type, IkBNS-deficient, and c-REL–deficient mice, and stimulated with 10 ng/ml PMA and 1 mM ionomycin for 8 h. ChIP was performed with anti–c-REL Ab, anti-IkBNS Ab, and control rabbit IgG (ctr IgG). Input chromatin and water were used as positive and negative controls, respectively. The experiment shown is representative of two independent experiments.
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    Image Search Results


    FIGURE 2. Normal expression and function of c-REL and IkBNS in the absence of the other factor. (A) Western blot analysis of NF-kB activation in anti- CD3 stimulated (10 mg/ml) total cells from peripheral lymph nodes of wild-type, IkBNS-deficient, c-REL–deficient, and DKO mice. Expression of CARMA1, c-REL, and IkBNS was analyzed along with NF-kB activation via the presence of phosphorylated and total IkBa. The experiment shown is representative of two independent experiments. (B) CD4+ T cells were purified by FACS, expanded for 7 d in vitro, and stimulated with 1 mM ionomycin and 10 ng/ml PMA for 4 h. Subsequently, nuclear and cytoplasmic fractions were analyzed for the presence of c-REL and IkBNS by Western blotting. ERK and LaminB served as controls for loading and fraction purity. The experiment shown is representative of three independent experiments. (C) CD4+ T cells were isolated from lymph nodes and spleens of wild-type, IkBNS-deficient, and c-REL–deficient mice, and stimulated with 10 ng/ml PMA and 1 mM ionomycin for 8 h. ChIP was performed with anti–c-REL Ab, anti-IkBNS Ab, and control rabbit IgG (ctr IgG). Input chromatin and water were used as positive and negative controls, respectively. The experiment shown is representative of two independent experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: c-REL and IκB NS Govern Common and Independent Steps of Regulatory T Cell Development from Novel CD122-Expressing Pre-Precursors.

    doi: 10.4049/jimmunol.1600877

    Figure Lengend Snippet: FIGURE 2. Normal expression and function of c-REL and IkBNS in the absence of the other factor. (A) Western blot analysis of NF-kB activation in anti- CD3 stimulated (10 mg/ml) total cells from peripheral lymph nodes of wild-type, IkBNS-deficient, c-REL–deficient, and DKO mice. Expression of CARMA1, c-REL, and IkBNS was analyzed along with NF-kB activation via the presence of phosphorylated and total IkBa. The experiment shown is representative of two independent experiments. (B) CD4+ T cells were purified by FACS, expanded for 7 d in vitro, and stimulated with 1 mM ionomycin and 10 ng/ml PMA for 4 h. Subsequently, nuclear and cytoplasmic fractions were analyzed for the presence of c-REL and IkBNS by Western blotting. ERK and LaminB served as controls for loading and fraction purity. The experiment shown is representative of three independent experiments. (C) CD4+ T cells were isolated from lymph nodes and spleens of wild-type, IkBNS-deficient, and c-REL–deficient mice, and stimulated with 10 ng/ml PMA and 1 mM ionomycin for 8 h. ChIP was performed with anti–c-REL Ab, anti-IkBNS Ab, and control rabbit IgG (ctr IgG). Input chromatin and water were used as positive and negative controls, respectively. The experiment shown is representative of two independent experiments.

    Article Snippet: For detection anti–c-REL (sc-71; Santa Cruz Biotechnology), anti-CARMA1/ CARD11 (mAb #4435; Cell Signaling Technology, Danvers, MA), antiIkBa (sc-371; Santa Cruz Biotechnology), anti–phospho-IkBa (mAb #9246; Cell Signaling Technology), and anti–b-actin (A2228; SigmaAldrich, Munich, Germany) Abs were used.

    Techniques: Expressing, Western Blot, Activation Assay, In Vitro, Isolation, Control